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Brian, yes sir, they both use .45 Colt (or .45 Long Colt) ammunition. Stay safe & have a great day!

Hi Brian and welcome. Yes Sir, this pistol is also chambered in .45 Long Colt so fires the same ammo as your Uberti 45 yellow boy 20 inch barrel. Cheers and stay safe!

Brian, it sure does. Both use 45 long Colt. So any ammo you have for your Uberti 45 yellow boy will also work in this. Hope this helps.